The following conditions may indicate a problem with the cytometer or the validation beads. Microbe counts and concentrations can be calculated directly and automatically from bd accuri c6 software statistics tables without the addition of counting beads. Even new flow cytometry users find it so easy to learn and use that. Even novice flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour.
Bd accuri c6 flow cytometry system available for sale. Special download site for pucl software purdue university. This is to ensure that your files will run on this software. Instrument includes a csampler, computer, software, validation report and warranty. Bd accuri c6 plus software files can be exported in fcs 3. However, you should also consult your flow core manager for their recommendation. I have recently developed cloud based flow cytometry software that will work on any device. Bd accuri c6 flow cytometer from bd biosciences selectscience. Its intuitive software coupled with its myriad visualization modes make for an easytouse but comprehensive data acquisition and analysis instrument. Facsdiva v6 software overview course bd biosciences accuri c6 software user guide.
The bd accuri c6 is a digital basic flow cytometer, which is equipped with a blue 488nm and a red 640nm lasers, two light scatter detectors fsc and ssc, and four fluorescence detectors. The aim of the present study was to evaluate the performance of the lowcost and easy to use accuri c6 flow cytometer becton dickinson, bd, franklin lakes, nj, usa, in comparison with that of two validated flow cytometers routinely used in our laboratory. Bd biosciences bd accuri c6 plus community, manuals and. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. Bd accuri c6 flow cytometer instrument manual this section provides details on installing bd accuri c6 software and the bd accuri c6 flow cytometer driver.
Bd accuri c6 software has an intuitive user interface that was developed based on hundreds of hours observing researchers using flow cytometers. Ive recently started doing flow cytometry bd accuri c6 in a core facility at my university, but my lab doesnt have any softtware to. Microbe counts and concentrations can be calculated directly and automatically from bd accuri c6 software statistics tables without the. The accuri c6 offers a budget friendly option for a bench top flow cytometer. Turn on the computer and log in with your ucf info. A guide to accuri c6 software published july 7, 2015 a new lab toy to make it big in the last 510 years is the accuri c6 cytometer now under the bd umbrella, a lowcost instrument in comparison to the big boys. We have also added some demonstrations of commercial packages. Nov 09, 2017 bd accuri c6, flow cytometry made easy. Selecting the optimal cytometer for your experiment can be as important as the panel you design. Bd accuri c6 cytometer manual getting ready ensure that the fluid levels in the sheath, cleaning, and decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. Bd accuri c6 plus software features an intuitive user interface. All fluorescence signals of labeled spermatozoa were analyzed by the bd accuri c6 flow cytometer and c6 plus flow cytometer becton dickinson, san jose, ca, usa. Can anyone recommend a good free facs analysing software. Bd accuri c6 system 32bit software is not forward compatible with bd accuri c6 plus 64bit software and the fcs 3.
Can anyone suggest good free flow cytometry software that can read the ncf. Bd accuri c6 cytometer manual getting ready ensure that the fluid levels in the sheath, cleaning, and decontamination bottles are sufficient. The c6 uses two lasers, red and blue, to record events and has 4 fluorescence channels for a wide range of fluorescence detection. Bd biosciences accuri c6 personal flow cytometer overview. Catalog of free flow cytometry software purdue university. Service for bd accuri c6 systems and their related options will continue until december 31 st, 2021. All reagents and kits are compatible with both bd accuri c6 plus and accuri c6 flow cytometer systems. It also includes common troubleshooting help and service information. Software options and instrument controls are clearly visible from the softwares tabbed interface.
The bd accuri c6 flow cytometer reports arithmetic means. Reflecting the input of hundreds of researchers, the bd accuri c6 flow cytometer is a fullfeatured benchtop cell and bead analysis system that provides similar capabilities to those of industryleading flow cytometers in an easytouse format and at a fraction of their cost. Discount is nontransferable and cannot be combined with other offers. By controlling all steps in highthroughput flow cytometry, users can quickly and easily create multiplate experiments, acquire data, and perform indepth analysis on thousands of samples all in one software platform. Feb 20, 2017 bd accuri c6 flow cytometry system available for sale. The bd accuri c6 flow cytometer system includes bd accuri c6 software for controlling the instrument, generating statistics, and analyzing results. It requires specific license document which will be. As a result, most novice flow cytometry users find it so easy to use, that they can collect and analyze data in less than an hour.
This portable and compact system holds the analytical power of a cytometer twice its size. When sample collection is completed, clean the sip and fluidics lines by following the procedure below. However, the accompanying software isnt the best, but it is free to use and does the job. The accuri c6 flow cytometer does not produce aerosols during normal operation. The bd accuri c6 plus offers simplified workflows and readytouse templates for performing a wide range of applications in flow cytometry.
Promotional pricing for the standard bd accuri c6 plus flow cytometer or the standard bd accuri c6 plus flow cytometer with the bd csampler plus is only valid if one extra year of warranty is also purchased. A total of 10 000 spermatozoa was counted with fluidic set at slow with flow rate of c6 plus. Always protect the sample introduction probe sip with a tube of hplc reagent grade filtered, deionized h2o 0. Csampler in order to acquire data, generate statistics, and analyze results. Since the initial commercialization of flow cytometry fc and fluorescence activated cell sorting facs in 1968, they have undergone significant improvements. Flow cytometry within reach the analytical power and versatility of todays laserbased flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. Instrument includes a c sampler, computer, software, validation report and warranty. Stratedigms automation suite is designed based on the same principles as our flow cytometers innovative, compact, and flexible. Critical evaluation of two models of flow cytometers for. By controlling all steps in highthroughput flow cytometry, users can quickly and easily create multiplate experiments, acquire data, and perform indepth analysis on thousands of samples all in one software. In bd accuri c6 software, the range of axes viewed is set using the zoom. The bd accuri c6 flow cytometer along with the bd cytometric bead array reagent sets, provides researchers a powerful flow cytometry toolset for cell analysis. Take a leap in flow cytometry with the bd accuri c6 plus. The bd accuri c6 flow cytometer is easy to use and allows student access to the science of flow cytometry.
Bd has discontinued selling the bd accuri c6 flow cytometer as of december 31, 2016. In most cases, bd accuri c6 software will be preinstalled. Displaying accuri cflow data and using the cflow importer in fcs. The bd facslyric system combines speed, simplicity and automation to help your laboratory be more productive. Flow cytometer automation stratedigm flow cytometers. It requires specific license document which will be prepared for each user. Bd csampler software controls the bd accuri c6 flow cytometer system with bd. The bd accuri c6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. On the bd accuri c6 flow cytometer, triggers and thresholds help to accurately identify small particles such as microbes. It has a very easy set up and the software interface is easy to navigate, however the analysis using the cflow software is a little. Cited in over 9,000 publications, the bd accuri c6 plus enables you to go beyond your current research potential. We have compiled this useful list of popular cytometers for your reference. This powerful flow cytometer and userfriendly bd facsuite software, along with a broad range of reagents and our history of service and quality, provide a truly integrated solution.
The accuri c6 flow cytometer is an increasingly popular flow cytometer. This manual provides information on cytometer requirements and specifications, setup, and maintenance. If you have questions or require further clarification, see the bd accuri c6 flow cytometer instrument manual or contact accuri technical support. Breakthrough with the bd accuri c6 plus cell analyzer. To provide free, user friendly and effective analysis tool for flow cytometry community. The accuri c6 is a compact flow cytometer with a wide dynamic range of over 7 decades. To develop new innovations and ideas for the analysis. The software that comes with the flow cytometer or facs should be used for controlling the instrument unless there. Flow cytometer flow cytometry systems flow cytometry is a laser based technology used to count and sort cells, detect biomarkers and engineer proteins, by suspending cells in a stream of fluid and passing them by an electronic detection system.
A practical guide for using flow cytometry and cell sorting, including extensive discussion on hardware, suppliers, reagents, and software. Using the bd accuri c6 cytometer for rapid and accurate. Bd accuri c6 flow cytometer quick set up guide please follow these directions for setting up your bd accuri c6 flow cytometer. This catalog strives to be comprehensive to include as much free flow cytometry software as possible. Any one have good or bad experiences with the new bd accuri c6 plus. The default configuration of the bd accuri c6 is similar to the facscalibur2 3blue 1red with filters optimized for the detection of fluorochromes such as fitc, pe. It has a very easy set up and the software interface is easy to navigate, however the analysis using the. Place a tube with 2 ml of filtered, deionized water on the sip and advance to any empty data well. Performance validation of the bd accuri c6 was then done. Jun 08, 2018 the accuri c6 offers a budget friendly option for a bench top flow cytometer. The attune nxt flow cytometer offers customizable configurations and can be set up with the attune nxt autosampler and integrated robotics. Bd accuri c6 software user guide manual 782009501 rev0 27. Bd biosciences accuri c6 personal flow cytometer software. Fcs files from flow cytometers with 18bit or higher digital signal processing, such as the bd accuri c6 flow cytometer system, often require adjustment of flowjos scaling factors to properly visualize the data.
The bd accuri c6 plus personal flow cytometer is the newest generation of the bd accuri platform. Description introducing the next generation bd accuri personal flow cytometer. Accuri c6 software automatically displays the percentage of cells within the region. Bd accuri c6 software controls the bd accuri c6 flow cytometer system in order to acquire data, generate statistics, and analyze results. Flow cytometry the bd accuri c6 flow cytometer was switched on and allowed to warm up for 15 minutes before analyses were begun. We frequently request a data set from you to test the program prior to giving you the software. Within 5 minutes of completing the water run, place a calibration sample on. Designed for all types of research, this instrument fits into any workflow from cell counts to screens for multicolor flow cytometric endpoint analysis. Bd biosciences accuri c6 personal flow cytometer features.
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